Dispase (Neutral Protease)
Referencia D3760-50mg
embalaje : 50mg
Marca : US Biological
D3760 Dispase (Neutral Protease)
Clone Type
PolyclonalGrade
Highly PurifiedShipping Temp
Blue IceStorage Temp
-20°C
I.U.B.: 3.4.24.28
A metallo, neutral protease exhibits mild proteolytic action that makes the enzyme especially suitable for the preparation of primary and secondary (subcultivation) cell culture. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications.
Unit Definition:
One Unit releases Folin positive amino acids equivalent to 1umole tyrosine per minute from casein at 37°C, pH 7.5.
Specific Activity:
≥4 units per mg dry weight
Protein:
As Reported
Units/mg Protein:
As Reported
Caseinase:
As Reported
Absorbance A280:
As Reported
Endotoxin:
As Reported
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.