Enhanced Antifade Mounting Medium with DAPI
Referencia MBS9719255-50mL
embalaje : 50mL
Marca : MyBiosource
Figure. Enhanced Antifade Mounting Medium with DAPI on paraffin sections of mouse lung tissue. Primary antibody CD34 (Cat #: MBS9700661, dilution ratio 1:200), Dylight 594, Goat Anti-Rabbit IgG (Cat #: MBS9718769, dilution ratio 1:200).
DAPI Staining Reagent – Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9719255Description: Enhanced Antifade Mounting Medium with DAPI is compatible with a wide range of fluorescent dyes and protects the entire visible and infrared spectrum from fluorescence signal quenching.Background: The irreversible destruction of fluorescent… More details
Product Overview
Online Datasheet in PDFProduct Name
Enhanced Antifade Mounting Medium with DAPI, Staining Reagent
Full Product Name
Enhanced Antifade Mounting Medium with DAPI
Product Gene Name
DAPI staining reagent
Product Synonym Names
Antifade Mounting Medium; Anti-fluorescence quenching agent; Antifade Mounting Medium with dapi; dapi; Antifade Mounting Medium with dapi; Anti-fluorescence quenching agent with dapi; BMU; BMU107
Preparation and Storage
Store at -20 degree C for 12 months, protected from light.
Features & Benefits
Strong anti-fluorescence quenching effect: Stored away from light for more than 2 weeks, the original luminous intensity can still be maintained.
High compatibility: The carefully optimized formula is compatible with a variety of fluorescent dyes.
Easy to operate: Ready-to-use, no configuration required.
High compatibility: The carefully optimized formula is compatible with a variety of fluorescent dyes.
Easy to operate: Ready-to-use, no configuration required.
Assay Procedure
I. Adherent cells:
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the glass slide, coat the cover glass with the cells, and let the cells contact the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the cell samples under the fluorescence microscope.
II. Tissue slices:
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the tissue slice, coat it with a cover glass, let the slice touch the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the tissue slices under the fluorescence microscope.
III Other samples:
For other samples, refer to cell samples or tissue slices for manipulation.
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the glass slide, coat the cover glass with the cells, and let the cells contact the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the cell samples under the fluorescence microscope.
II. Tissue slices:
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the tissue slice, coat it with a cover glass, let the slice touch the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the tissue slices under the fluorescence microscope.
III Other samples:
For other samples, refer to cell samples or tissue slices for manipulation.
Product Categories/Family for DAPI staining reagent
Protein General Reagents; Cell Staining/Tracing Kit; Protein
Applications Tested/Suitable for DAPI staining reagent
Immunohistochemistry (IHC)
Application Notes for DAPI staining reagent
Strawberry moment: In order to prolong the fluorescence quenching time, in addition to using anti-fluorescence quenching agent to prevent fluorescence quenching, the stability of antibody and fluorescence itself are also very important.
Precautions:
1. Fluorescent substances are prone to quenching, so stained samples should be stored away from light.
2. In the case of using Enhanced Antifade Mounting Medium with DAPI, quenching can be slowed down, but it is still advisable to avoid light and take pictures as soon as possible
Precautions:
1. Fluorescent substances are prone to quenching, so stained samples should be stored away from light.
2. In the case of using Enhanced Antifade Mounting Medium with DAPI, quenching can be slowed down, but it is still advisable to avoid light and take pictures as soon as possible
Other Notes
Small volumes of DAPI staining reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DAPI purchase
MBS9719255 is a staining reagent targeting DAPI. Also known as Enhanced Antifade Mounting Medium with DAPI.