Fibrillarin antibody [38F3]
Referencia GTX24566-250ul
embalaje : 250ul
Marca : Genetex
Contact local distributor :
Teléfono : +1 850 650 7790
![Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | 38F3 |
Isotype | IgG1 |
Application | WB, ICC/IF, IHC-P, IHC |
Reactivity | Human, Mouse, Rat, Yeast, Drosophila, Caenorhabditis elegans, Schizosaccharomyces pombe, Plant |
| Package | 250 μl |
Datasheet File View product citations for antibody GTX24566 on CiteAb
APPLICATION
Application Note
IF (ICC): 1/1000 - 1/5000 (yeast cells). For IF of mammalian cells, 1/500. WB: 1/2000 (cell lysates) - 1/10000 (nuclear fractions)(ECL). Detects a band of approximately 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000. To detect mammalian fibrillarin on western blots by ECL, 1/500. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
34 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
Tissue culture supernatant
Preservative
10mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Yeast nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_367009
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibrillarin , FIB , FLRN , Nop1 , RNU3IP1
Cellular Localization
Nucleolus
Background
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
| | GTX24566 ICC/IF ImageHuman neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000 |
| | GTX24566 ICC/IF ImageHigh magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixat |
| | GTX24566 IHC ImageRat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). |
| | GTX24566 WB ImageBlot of yeast protein extract stained with the GTX24566 anti-Gibrillarin anitbody, stained a single band at ~34kDa. |
Application Reference
Bennett CL et al. Heliyon 2020; 6 (6) : e04165 Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.
Xiangduo Kong et al. bioRxiv 2019; (Epub) The cohesin loader NIPBL interacts with pre-ribosomal RNA and treacle to regulate ribosomal RNA synthesis.
Application : ICC/IF
Reactivity : Human
Behm M et al. J Cell Sci 2017; 130 (4) : 745-753 Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
Application : ICC/IF
Reactivity : Mouse
Zougman A et al. Nucleic Acids Res 2011; 39 (16) : 7103-13 Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.
Application : ICC/IF
Reactivity : Human
REVIEW
Application Reference
Bennett CL et al. Heliyon 2020; 6 (6):e04165 Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.
Xiangduo Kong et al. bioRxiv 2019; (Epub) The cohesin loader NIPBL interacts with pre-ribosomal RNA and treacle to regulate ribosomal RNA synthesis.
Application : ICC/IF
Reactivity : Human
Behm M et al. J Cell Sci 2017; 130 (4):745-753 Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
Application : ICC/IF
Reactivity : Mouse
Zougman A et al. Nucleic Acids Res 2011; 39 (16):7103-13 Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.
Application : ICC/IF
Reactivity : Human
| SDS | |
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| Sodium Azide.pdf | |