FlyCut^® SalI is expressed and purified from E.coli that carries the recombinant SalI gene. The molecular weight is 36.3 kDa, with the recognition site at G^TCGAC The reaction is conducted at 37oC, and heat-inactivated at 65^oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.

Enzyme Properties 

· Fast digestion in 5 minutes

· Universal buffer

· Star activity-free

Application 

Genomic DNA, plasmid DNA, PCR product 

Storage

at -20^oC for two years

Shipping

Dry ice (-70℃)