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> Formic acid (ammonium) [540-69-2]

Formic acid (ammonium) [540-69-2]

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Pedido mínimo 2

Referencia HY-Y0488A-1mL

embalaje : 10mM/1mL

Marca : MedChemExpress


Descripciòn

Formic acid ammonium is the ammonium salt form of formic acid, which is an endogenous metabolite. Formic acid ammonium activates the nitric oxide/cyclic guanosine monophosphate (NO/cGMP) signaling pathway in endothelial cells and inhibits calcium ion influx in vascular smooth muscle cells, resulting in vasomotor relaxation. Formic acid ammonium inhibits cytochrome C oxidase (CcO) and disrupts the electron transport chain, leading to ATP depletion. Formic acid ammonium can be used as a feed additive, which can lower the feed pH value, inhibit the growth of microorganisms (such as Escherichia coli, Salmonella), and extend the shelf life of the feed[1][2][3].

In Vitro

Formic acid ammonium (0.1-10 mM) induces vasodilation in large isolated thoracic aortic rings in a concentration-dependent manner with EC₅₀ values in endothelium-intact and endothelium-deprived vascular rings of 2.315 mM and 2.132 mM, respectively[2].
Formic acid ammonium (0.5-5 mM,2 h) upregulates the expression of eNOS in rat aortic tissue, enhances the activity of NOS, and increases the production of NO and cGMP[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Formic acid ammonium Related Antibodies

RT-PCR[2]

Cell Line: Ex vivo thoracic aortic ring
Concentration: 0.5, 1 and 5 mM
Incubation Time: 2h
Result: Significantly increased eNOS mRNA, and the mRNA levels of nNOS and iNOS remained unchanged.

Western Blot Analysis[2]

Cell Line: Ex vivo thoracic aortic ring
Concentration: 0.5, 1 and 5 mM
Incubation Time: 2h
Result: Significantly increased eNOS protein, and the protein levels of nNOS and iNOS remained unchanged.

ELISA Assay[2]

Cell Line: Ex vivo thoracic aortic ring
Concentration: 0.5, 1 and 5 mM
Incubation Time: 2h
Result: Significantly increased NO and cGMP, and the level of cAMP remained unchanged.
In Vivo

Formic acid ammonium has direct toxicity towards rat embryos, which can lead to abnormal development[3].
Formic acid ammonium induces reversible retinal toxicity in a methanol-induced visual toxicity rat model following short-term exposure[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Peso molecular

63.06

Fòrmula

HCO2NH4
No. CAS
Appearance

Solid

Color

Colorless to off-white

SMILES

12C(O[NH4])=O
Structure Classification
Initial Source
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Store at room temperature, keep dry and cool

In solvent -80°C 1 year
-20°C 6 months
Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (1585.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 15.8579 mL 79.2896 mL 158.5791 mL
5 mM 3.1716 mL 15.8579 mL 31.7158 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (79.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (79.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Pureza y Documentación
Referencias