FRK siRNA (Mouse)
Referencia MBS829764-30nmol
embalaje : 30nmol
Marca : MyBiosource
FRK siRNA – Tyrosine-Protein Kinase FRK (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829764MBS829764 is an siRNA targeting FRK. Also known as FRK siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit FRK expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
FRK, siRNA
Full Product Name
FRK siRNA (Mouse)
Product Gene Name
FRK sirna
Product Synonym Names
BSK; IYK; Tyrosine-protein kinase FRK; Beta-cell Src-homology tyrosine kinase; BSK; FYN-related kinase; Intestine tyrosine kinase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
FRK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
58,844 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FRK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for FRK sirna
RNA Interference (RNAi)
Other Notes
Small volumes of FRK sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for FRK. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tyrosine-protein kinase FRK
NCBI Official Synonym Full Names
fyn-related kinase
NCBI Official Symbol
Frk
NCBI Official Synonym Symbols
BSK; GTK; RAK; C85044; BSK/IYK
UniProt Protein Name
Tyrosine-protein kinase FRK
UniProt Synonym Protein Names
Beta-cell Src-homology tyrosine kinase; BSK; FYN-related kinase; Intestine tyrosine kinase
Protein Family
Tyrosine-protein kinase
UniProt Gene Name
Frk
UniProt Synonym Gene Names
Bsk; Iyk; BSK
UniProt Entry Name
FRK_MOUSE
NCBI Protein Information
tyrosine-protein kinase FRK
UniProt Comments for FRK
FRK: a tyrosine kinase of the Src family. Expressed in cell lines derived from tissues of lymphoid, brain, breast, colon and bladder origin. A nuclear protein that may function during G1 and S phase of the cell cycle to suppress growth.
Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Tumor suppressor; TK group; Src family
Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; nucleus
Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity; receptor binding
Biological Process: cell migration; innate immune response; cell differentiation; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation
Show more Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Tumor suppressor; TK group; Src family
Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; nucleus
Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity; receptor binding
Biological Process: cell migration; innate immune response; cell differentiation; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with FRK sirna
| Reference Product | PubMed Publications |
|---|---|
| Pten sirna | >4 publications with FRK and Pten |
| Cttn sirna | >1 publications with FRK and Cttn |
| Bcar1 sirna | >1 publications with FRK and Bcar1 |
Diseases associated with FRK sirna
| Disease Name | Pubmed Publications |
|---|---|
| Breast Neoplasms Antibodies | >23 publications with FRK and Breast Neoplasms |
| Adenocarcinoma Antibodies | >9 publications with FRK and Adenocarcinoma |
| Ovarian Neoplasms Antibodies | >8 publications with FRK and Ovarian Neoplasms |
| Nervous System Diseases Antibodies | >8 publications with FRK and Nervous System Diseases |
| Neoplasms, Experimental Antibodies | >5 publications with FRK and Neoplasms, Experimental |
| Vascular Diseases Antibodies | >5 publications with FRK and Vascular Diseases |
| Liver Neoplasms Antibodies | >5 publications with FRK and Liver Neoplasms |
| Cell Transformation, Neoplastic Antibodies | >5 publications with FRK and Cell Transformation, Neoplastic |
| Necrosis Antibodies | >5 publications with FRK and Necrosis |
| Carcinoma, Hepatocellular Antibodies | >4 publications with FRK and Carcinoma, Hepatocellular |
Organs/Tissues associated with FRK sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Kidney Antibodies | >10 publications with FRK and Kidney |
| Heart Antibodies | >9 publications with FRK and Heart |
| Ovary Antibodies | >9 publications with FRK and Ovary |
| Lung Antibodies | >8 publications with FRK and Lung |
| Prostate Antibodies | >7 publications with FRK and Prostate |
| Pancreas Antibodies | >6 publications with FRK and Pancreas |
| Embryonic Tissue Antibodies | >6 publications with FRK and Embryonic Tissue |
| Intestine Antibodies | >4 publications with FRK and Intestine |
| Mammary Gland Antibodies | >4 publications with FRK and Mammary Gland |
| Skin Antibodies | >3 publications with FRK and Skin |