G6PD ELISA Kit (Human)
Referencia OKCD06718
embalaje : 96Wells
Marca : Aviva Systems Biology
G6PD ELISA Kit (Human) (OKCD06718)
| Datasheets/Manuals | Printable datasheet for G6PD ELISA Kit (Human) (OKCD06718) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose-6-phosphate Dehydrogenase (G6PD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose-6-phosphate Dehydrogenase (G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose-6-phosphate Dehydrogenase (G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glucose-6-phosphate Dehydrogenase (G6PD) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose-6-phosphate Dehydrogenase (G6PD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose-6-phosphate Dehydrogenase (G6PD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 0.267ng/mL |
| Gene Symbol | G6PD |
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| Gene Full Name | glucose-6-phosphate dehydrogenase |
| Alias Symbols | epididymis secretory sperm binding protein;G6PD1;glucose-6-phosphate 1-dehydrogenase. |
| NCBI Gene Id | 2539 |
| Protein Name | Glucose-6-phosphate 1-dehydrogenase |
| Description of Target | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. |
| Uniprot ID | P11413 |
| Protein Accession # | NP_000393.4 |
| Nucleotide Accession # | NM_000402.4 |
