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> Gibberellic acid [77-06-5]

Gibberellic acid [77-06-5]

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Referencia HY-N1964-1ml

embalaje : 10mM/1mL

Marca : MedChemExpress

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Descripciòn

Gibberellic Acid is named after the fungus Gibberella fujikuroi. Gibberellic Acid regulates processes in plant development and growth, including seed development and germination, stem and root growth, cell division, and flowering time. Gibberellic Acid also improves plant response to growth stress caused by various environmental stresses, such as cold stress, drought stress, heavy metal stress, etc. Gibberellic Acid also causes increased lipid peroxidation and fluctuations in the antioxidant defense system in rats[1][2][3][4].

Cellular Effect
Cell Line Type Value Description References
CA46 IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human CA46 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human CA46 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
HCC1599 IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human HCC1599 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human HCC1599 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
HEK-293T IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human HEK293T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human HEK293T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
Hs-578T IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human Hs-578T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human Hs-578T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
NCI-H446 IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
PSN1 IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human PSN1 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human PSN1 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
RCK8 IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human RC-K8 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human RC-K8 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
SUD4 IC50
> 10000 nM
Compound: JRA-001
Cytotoxicity against human SU-DHL-4 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human SU-DHL-4 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
[PMID: 33062173]
In Vitro

Gibberellic acid rescues chickpea seedlings from cold stress. Gibberellic acid (10-20 μM) increases final emergence probability (FEP), helping seeds maintain higher relative water content and lower electrolyte leakage[2].
Gibberellic acid (0.025 μM) treatment of Echinacea purpurea L. hairy roots increased PAL activity, cell viability, and free radical scavenging activity in hairy root cultures. Gibberellic acid optimizes the production of secondary metabolites in Echinacea purpurea L. hairy root cultures and is beneficial to the growth of hairy roots[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Gibberellic acid Related Antibodies

Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (288.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 4 mg/mL (11.55 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8871 mL 14.4354 mL 28.8709 mL
5 mM 0.5774 mL 2.8871 mL 5.7742 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
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