ASM10213-A633-100 | GluN2B/NR2B Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (ASM10213). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (ASM10213) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.
Western Blot analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (ASM10213). Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (ASM10213) at 1:250.
Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (ASM10213). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (ASM10213) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.
Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (ASM10213). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (ASM10213) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC, IP, AM
Primary Accession	Q00960
Other Accession	NP_036706.1
Host	Mouse
Isotype	IgG2b
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2b
Target/Specificity	Detects ~166kDa. No cross-reactivity against NR2A.
Other Names	NMDAR2B Antibody, hNR3 Antibody, MGC142180 Antibody, glutamate receptor subunit epsilon-2 Antibody, NR3 Antibody
Clone Names	S59-36
Immunogen	Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-337 was sufficient for detection of NR2B in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cell Membrane \| Cell Junction \| Synapse \| Postsynaptic Cell Membrane

GluN2B/NR2B Antibody