Goat Thyroid Stimulating Hormone,TSH ELISA Kit
Referencia CSB-E13278G-24T
embalaje : 24T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
glycoprotein hormones, alpha polypeptide
Associated names
CGAGlycoprotein hormones alpha chain ELISA Kit; Anterior pituitary glycoprotein hormones common subunit alpha ELISA Kit; Follicle-stimulating hormone alpha chain ELISA Kit; FSH-alpha ELISA Kit; Follitropin alpha chain ELISA Kit; Luteinizing hormone alpha chain ELISA Kit; LSH-alpha ELISA Kit; Lutropin alpha chain ELISA Kit; Thyroid-stimulating hormone alpha chain ELISA Kit; TSH-alpha ELISA Kit; Thyrotropin alpha chain ELISA Kit
Abbreviation
Uniprot No.
Species
Capra hircus (Goat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.5 μIU/mL-20 μIU/mL
Sensitivity
0.25 μIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of goat TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-98 | |||||
Recovery
| The recovery of goat TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-97 | ||||
| EDTA plasma (n=4) | 105 | 102-109 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Goat CGA ELISA Kit was designed for the quantitative measurement of Goat CGA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.5 μIU/mL-20 μIU/mL and the sensitivity is 0.25 μIU/mL.
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Target Background
Function
(From Uniprot)
(From Uniprot)
Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH and follitropin/follicle stimulating hormone/FSH. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit alpha family
Database Links
KEGG:
UniGene: