GST Colorimetric Activity Assay Kit

Glutathione S-transferase (GST) is a group of enzymes that involved in the detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobitocs. It utilizes glutathione to scavenge toxic substances that produced by oxidative stress. The GST Colorimetric Activity Assay Kit is rely on the GST-catalyzed reaction of GSH and the GST substrate-CDNB (1-chloro-2,4-dinitrobenzene). A dinitrophenyl thioether is produced by GST-catalyzed formation of GS-DNB. It can be detected by spectrophotometer at 340 nm. The kit can detect GST activity in crude cell lysate, purified protein fraction and quantitate GST-tagged fusion protein.

Figure 1: Typical GST kinetic assay curve for sample (left) and background (right)
GST activity = (0.1093-0.0067)*0.036*100/0.002 =185 mU/mL