Hamster C-reactive protein(CRP) ELISA Kit
Referencia CSB-EL005991HA-24T
embalaje : 24T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
C-reactive protein(CRP)
Associated names
CRP ELISA kit; PTX1C-reactive protein ELISA kit
Abbreviation
Uniprot No.
Species
Mesocricetus auratus (Golden hamster)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 ng/mL-800 ng/mL
Sensitivity
3.12 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:10 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 94-104 | |||||
Recovery
| The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-93 | ||||
| EDTA plasma (n=4) | 96 | 87-100 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Hamster CRP ELISA Kit was designed for the quantitative measurement of Hamster CRP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.
Citations
- N Laham-Karam, P Mäkinen, E Koivulehto, E Gurzeler,npj Vaccines,2026
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Target Background
Function
(From Uniprot)
(From Uniprot)
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Subcellular Location
Secreted.
Protein Families
Pentraxin family
Tissue Specificity
Found in plasma.