HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

Specifications

Product Data
Clone Name	W6/32
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 μl of neat antibody to label 1x106 cells or 100 μl whole blood. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Reactivities	Bovine, Feline, Human, Monkey, Primate
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Purified human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the NS1/1 - Ag4.1 mouse myeloma cell line.
Specificity	Antibody SM1222R recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (See Ishitani, A. et al. for details). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.
Formulation	PBS Label: PE State: Lyophilized purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1ml distilled water
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Background	The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Synonyms	MHC class I ABC, HLA Class 2 ABC
Reference Data