Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> HSP90 beta Antibody

HSP90 beta Antibody

Imprimir Imprimir
Por favor conéctese

Referencia ASM10432-A655-100

embalaje : Onrequest

Marca : Abcepta

Contact local distributor :


Teléfono : +1 850 650 7790

 
  • ICC/IF - HSP90 beta Antibody ASM10432
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 beta Polyclonal Antibody (ASM10432). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (ASM10432) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 beta Antibody. (C) Composite.
  • WB - HSP90 beta Antibody ASM10432
    Western blot analysis of Human Cell line lysates showing detection of HSP90 beta protein using Rabbit Anti-HSP90 beta Polyclonal Antibody (ASM10432). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 beta Polyclonal Antibody (ASM10432) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Left: 2 ug of Hsp90 Alpha, Right: 2 ug Hsp90 Beta.
  • IHC - HSP90 beta Antibody ASM10432
    Immunohistochemistry analysis using Rabbit Anti-HSP90 beta Polyclonal Antibody (ASM10432). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 beta Polyclonal Antibody (ASM10432) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
  • ICC/IF - HSP90 beta Antibody ASM10432
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 beta Polyclonal Antibody (ASM10432). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 beta Polyclonal Antibody (ASM10432) at 1:120 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 beta Antibody. (C) Composite.
  • WB - HSP90 beta Antibody ASM10432
    Western blot analysis of Human Cell line lysates showing detection of HSP90 beta protein using Rabbit Anti-HSP90 beta Polyclonal Antibody (ASM10432). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 beta Polyclonal Antibody (ASM10432) at 1:2000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF
Primary Accession P08238
Other Accession NP_031381.2
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human HSP90 beta Polyclonal
Target/Specificity Detects ~90kda. Does not cross-react with HSP90α.
Other Names HSP84 Antibody, HSP90 Antibody, HSP86 Antibody, HSP89 Antibody, HSP90B Antibody, HSP90BETA Antibody, HSP90N Antibody, HSPC2 Antibody, HSPCA Antibody, HSPCAL1 Antibody, HSPCB Antibody, HSPN Antibody, LAP2 Antibody, NY REN 38 antigen Antibody
Immunogen Full length protein HSP90
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-177 was sufficient for detection of HSP90 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization Cytoplasm | Melanosome