Human EchoVirus IgG (ECHO-IgG) ELISA Kit

An ECHO (enteric cytopathic human orphan) virus is a type of RNA virus that belongs to the species Enterovirus B, genus Enterovirus of the Picornaviridae family. Echoviruses are found in the gastrointestinal tract (hence it being part of the enterovirus genus) and exposure to the virus causes other opportunistic infections and diseases.
Echovirus is highly infectious, and its primary target is children. The echovirus is among the leading causes of acute febrile illness in infants and young children, and is the most common cause of aseptic meningitis. Infection of an infant with this virus following birth may cause severe systemic diseases, and is associated with high infant mortality rates. The echovirus can mimic symptoms caused by other common bacterial and viral infections.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ECHO-IgG in samples. An antibody specific for ECHO-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyECHO-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECHO-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECHO-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ECHO-IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283928 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EchoVirus IgG (ECHO-IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ECHO-IgG. The ELISA analytical biochemical technique of the MBS283928 kit is based on ECHO-IgG antibody-ECHO-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ECHO-IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ECHO-IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).