General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This human FABP2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | FABP2 |
| Gene ID: | 2169 |
| Uniprot ID: | FABPI_HUMAN |
| Specificity: | This kit recognizes Human IFABP/FABP2 in samples. No significant cross-reactivity or interference between Human IFABP/FABP2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Tissue Specificity | Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. |
| Function | FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. |
| Protein Name | Fatty Acid-Binding Protein - IntestinalFatty Acid-Binding Protein 2Intestinal-Type Fatty Acid-Binding ProteinI-Fabp |
| Database Links | Reactome: R-HSA-163560 |
| Cellular Localisation | Cytoplasm |
| Alternative CLIA Names | Fatty Acid-Binding Protein - Intestinal CLIA kitFatty Acid-Binding Protein 2 CLIA kitIntestinal-Type Fatty Acid-Binding Protein CLIA kitI-Fabp CLIA kitFABP2 CLIA kitFABPI CLIA kit |
| Specificity | This kit recognizes Human IFABP in samples. No significant cross-reactivity or interference between Human IFABP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 94 |
| EDTA plasma(n=8) | 97-114 | 104 |
| Cell culture media(n=8) | 99-113 | 104 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 109.71 | 245.91 | 875.57 | 116.29 | 236.29 | 822.75 |
| Standard deviation | 9.24 | 26.78 | 55.16 | 11.82 | 19.61 | 83.84 |
| CV (%) | 8.42 | 10.89 | 6.30 | 10.16 | 8.30 | 10.19 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance