Human FPR2 (N-formyl peptide receptor 2) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	FPR2
Gene ID:	2358
Uniprot ID:	FPR2_HUMAN
Specificity:	This kit recognizes Human FPR2 in samples. No significant cross-reactivity or interference between Human FPR2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Detected in lung, bone marrow, neutrophils, spleen and testis.
Function	Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophil chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of pro-inflammatory signals such as LTB4 (leukotriene B4). Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation. Acts as a receptor for humanin.
Protein Name	N-Formyl Peptide Receptor 2 Fmlp-Related Receptor I Fmlp-R-I Formyl Peptide Receptor-Like 1 Hm63 Lipoxin A4 Receptor Lxa4 Receptor Rfp
Database Links	Reactome: R-HSA-416476 Reactome: R-HSA-418594 Reactome: R-HSA-444473 Reactome: R-HSA-6798695
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Associates With Amyloid-Beta Protein 42 Product Of App At The Cell Surface And The Complex Is Then Rapidly Internalized Also Internalized In The Presence Of Humanin
Alternative CLIA Names	N-Formyl Peptide Receptor 2 CLIA kit Fmlp-Related Receptor I CLIA kit Fmlp-R-I CLIA kit Formyl Peptide Receptor-Like 1 CLIA kit Hm63 CLIA kit Lipoxin A4 Receptor CLIA kit Lxa4 Receptor CLIA kit Rfp CLIA kit FPR2 CLIA kit FPRH1 CLIA kit FPRL1 CLIA kit LXA4R CLIA kit
Specificity	This kit recognizes Human FPR2 in samples. No significant cross-reactivity or interference between Human FPR2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	99-116	107
EDTA plasma(n=8)	83-97	90
Cell culture media(n=8)	99-116	107

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	47.07	114.53	429.70	47.94	104.56	397.60
Standard deviation	4.52	11.11	44.86	4.52	7.47	28.43
CV (%)	9.60	9.70	10.44	9.43	7.14	7.15

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance