Orb1807496-48Tests | Human Granulocyte-Macrophage Colony Stimulating

Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) ELISA Kit

SKU: orb1807496

Description

Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GM-CSF. You can calculate the concentration of Human GM-CSF in the samples by comparing the OD of the samples to the standard curve.

Application Notes

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GM-CSF. You can calculate the concentration of Human GM-CSF in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Sandwich
Assay Time	3.5H
Range	7.82-500pg/mL
Sensitivity	3.1 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GMCSF, CSF2

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Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit [orb778165]
Reactivity:
Human
Dynamic Range:
0.32-20 ng/mL
Sensitivity:
0.113 ng/mL
Sizes Available: 48 T, 96 T
Human Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit [orb774893]
Reactivity:
Human
Dynamic Range:
15.63-1000 pg/mL
Sensitivity:
6.5 pg/mL
Sizes Available: 48 T, 96 T
Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) High Sensitivity ELISA Kit [orb1945903]
Reactivity:
Human
Dynamic Range:
0.78-50pg/mL
Sensitivity:
0.47 pg/mL
Sizes Available: 48 T, 96 T
Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) Microsample Fast ELISA Kit [orb1806767]
Reactivity:
Human
Dynamic Range:
7.81-500pg/mL
Sensitivity:
4.69 pg/mL
Sizes Available: 48 T, 96 T
Human GMCSF(Colony Stimulating Factor 2, Granulocyte Macrophage) Microsample ELISA Kit [orb2999408]
Reactivity:
Human
Dynamic Range:
15.63-1000 pg/mL
Sensitivity:
6.8 pg/mL
Sizes Available: 48 T, 96 T

Quick Database Links

Gene Symbol

GMCSF

UniProt

P04141

UniProt Details

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No UniProt data available

Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) ELISA Kit

Referencia orb1807496-48Tests

Marca : Biorbyt

Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit [orb778165]

Human Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit [orb774893]

Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) High Sensitivity ELISA Kit [orb1945903]

Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) Microsample Fast ELISA Kit [orb1806767]

Human GMCSF(Colony Stimulating Factor 2, Granulocyte Macrophage) Microsample ELISA Kit [orb2999408]

Quick Database Links

UniProt Details

Protocol Information

Reactivity:	Human
Dynamic Range:	0.32-20 ng/mL
Sensitivity:	0.113 ng/mL