RayBio® Human LBP ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Human LBP ELISA Kit
Human LBP ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.82 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.82 ng/ml - 200 ng/ml |
| Specificity | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM-12, ADAM-8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD229, CD48, CD58 (LFA-3), CD84, CD99, CEACAM-5, Coagulation Factor XIV (Protein C), Cystatin A, Cystatin B, Cystatin E/M, Desmoglein-2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin-3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4,Testican 2, TIM-3, TLR4, TRAIL, ULBP-1. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 1,000 fold |
| Accession Number | P18428 |
| Gene Id | 3929 |
| Gene Symbols | LBP |
| Protein Name / Synonyms | Lipopolysaccharide-binding protein (LBP) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Customize This Human LBP ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified LBP, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
The "Movie Theater" Study: Acute Cardiometabolic Effects of a Cinema-Style Meal
- Species:
- Human
- Sample Type:
- Serum (fasting and postprandial)
- Year:
- 2026
- Journal Name:
- Metabolites
- PubMed ID:
- 41745620
Children and adolescents with attention deficit hyperactivity disorder and autism spectrum disorder share distinct microbiota compositions
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Gut microbes
Postprandial Cardiometabolic Parameters in Older Adults with Normal-Weight Obesity: A Cross-Sectional Pilot Study
- Species:
- Human
- Sample Type:
- Serum (baseline and 4 h postprandial)
- Year:
- 2025
- Journal Name:
- Metabolites
- PubMed ID:
- 40863166
Associations of circulating levels of phthalate metabolites with cytokines and acute phase reactants in a Spanish human cohort
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Environmental Research
Differences in bacterial taxa between treatment-naive patients with major depressive disorder and non-affected controls may be related to a proinflammatory profile
- Species:
- Human
- Sample Type:
- Plasma (peripheral blood plasma)
- Year:
- 2024
- Journal Name:
- BMC psychiatry
- PubMed ID:
- 38297265
Intestinal permeability biomarkers in patients with schizophrenia: Additional support for the impact of lifestyle habits
- Species:
- Human
- Sample Type:
- Serum (schizophrenia patients and controls)
- Year:
- 2024
- Journal Name:
- European psychiatry : the journal of the Association of European Psychiatrists
- PubMed ID:
- 39676547
Persistent Systemic Microbial Translocation and Intestinal Damage During Coronavirus Disease-19
- Species:
- Human
- Sample Type:
- Plasma (COVID-19 patients)
- Year:
- 2021
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 34335624
Effects of Synbiotic Supplementation on Chronic Inflammation and the Gut Microbiota in Obese Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Study
- Species:
- Human
- Sample Type:
- Plasma (obese T2DM patients, RCT)
- Year:
- 2021
- Journal Name:
- Nutrients
- PubMed ID:
- 33567701
Higher Lipopolysaccharide Binding Protein and Chemerin Concentrations Were Associated with Metabolic Syndrome Features in Pediatric Subjects with Abdominal Obesity during a Lifestyle Intervention
- Species:
- Human
- Sample Type:
- Plasma (pediatric abdominal obesity)
- Year:
- 2021
- Journal Name:
- Nutrients
- PubMed ID:
- 33498461
Probiotics, Anticipation Stress, and the Acute Immune Response to Night Shift
- Species:
- Human
- Sample Type:
- Serum (night shift workers, probiotic trial)
- Year:
- 2020
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 33584665
