Human NAMPT (Nicotinamide phosphoribosyltransferase) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	NAMPT
Gene ID:	10135
Uniprot ID:	NAMPT_HUMAN
Specificity:	This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Function	Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.
Protein Name	Nicotinamide Phosphoribosyltransferase Namprtase Nampt Pre-B-Cell Colony-Enhancing Factor 1 Pre-B Cell-Enhancing Factor Visfatin
Database Links	Reactome: R-HSA-1368108 Reactome: R-HSA-197264 Reactome: R-HSA-9768919
Cellular Localisation	Nucleus Cytoplasm Secreted Under Non-Inflammatory Conditions Visfatin Predominantly Exhibits A Granular Pattern Within The Nucleus Secreted By Endothelial Cells Upon Il-1beta Stimulation Abundantly Secreted In Milk Reaching 100-Fold Higher Concentrations Compared To Maternal Serum
Alternative CLIA Names	Nicotinamide Phosphoribosyltransferase CLIA kit Namprtase CLIA kit Nampt CLIA kit Pre-B-Cell Colony-Enhancing Factor 1 CLIA kit Pre-B Cell-Enhancing Factor CLIA kit Visfatin CLIA kit NAMPT CLIA kit PBEF CLIA kit PBEF1 CLIA kit
Specificity	This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	100-114	107
EDTA plasma(n=8)	90-102	97
Cell culture media(n=8)	87-98	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	25.85	44.62	180.11	23.30	43.30	177.99
Standard deviation	2.80	5.27	20.42	2.15	4.14	15.91
CV (%)	10.83	11.81	11.34	9.23	9.56	8.94

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance