IgG2ab Antibody (TRITC)

Rat IgG2ab

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Tetramethyl Rhodamine Iso-Thiocyanate. Also available Unconjugated or conjugated with Biotin, FITC, HRP.

Purified IgG

Unmodified

Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat IgG2ab, subclass specific.

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to identify and measure IgG2 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.