K2078-40 assays | KinaseSTAR JNK Activity Screening Kit Clinisciences

Background

c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein (MAP) kinase family identified in mammals. Activation of JNK plays an critical role in neuronal apoptosis and other physiological and pathological processes.

The KinaseSTAR JNK Activity Screening Kit provides a simple and convenient way for screening of JNK activity based on Western Blot method. The assay uses an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell or tissue lysates. After washing to remove nonspecifically bound proteins, the kinase reaction is conduced in the presence of cold ATP. The phosphorylation of c-Jun is detected by Western blot method using a phospho-c-Jun specific antibody. The kit is suited for both research and drug discovery.

Features & Properties

Features	Simple procedure The kit is suitable for both research and drug discovery. The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate. c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.