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> Lactic acid (potassium) [996-31-6]

Lactic acid (potassium) [996-31-6]

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Referencia HY-B2227C

embalaje : 10mM/1mL

Marca : MedChemExpress


Descripciòn

Lactic acid (DL-Lactic acid) potassium is a hydroxycarboxylic acid receptor 1 (HCAR1) activator and an epigenetic modulator inducing lysine residues lactylation. Lactic acid potassium is a glycolysis end-product, bridging the gap between glycolysis and oxidative phosphorylation. Lactic acid potassium is an oncometabolite and has immune protective role of lactate in anti-tumor immunity[1][2]. Lactic acid also potassium has antimicrobial activity, which can be used as a food preservative[3].

IC50 & Target

Bacterial[2]
In Vitro

Lactic acid (20 mM; 24, 48 h) potassium results in a significant decrease in cell proliferation and migration, acting and switching cell metabolism toward oxidative phosphorylation[1].
Lactic acid (20 mM; 24 h) potassium shows an increase in the protein and mRNA expression levels of MCT1 and HCAR1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lactic acid potassium Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Uveal melanoma 92.1 cells
Concentration: 20 mM
Incubation Time: 24, 48 h
Result: Inhibited cell proliferation and migration.

Western Blot Analysis[1]

Cell Line: Uveal melanoma 92.1 cells
Concentration: 20 mM
Incubation Time: 24 h
Result: Showed an increase in the protein expression levels of MCT1 and HCAR1

RT-PCR[1]

Cell Line: Uveal melanoma 92.1 cells
Concentration: 20 mM
Incubation Time: 24 h
Result: Induced a significant increase in mRNA expression levels of SLC16A1 and HCAR1.
In Vivo

Lactic acid (1.6 g/kg; pH 7.4; subcutaneous; daily from Day 8 to 30 days) potassium shows CD8+ T cell-dependent tumor growth inhibition in MC38 colon cancer model[2].
Note: The pH of the working solution needs to be adjusted to around 7.4 before administration to animals.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6 mice with MC38 colon cancer cells[2]
Dosage: 1.6 g/kg
Administration: Subcutaneous; daily from Day 8 to 30 days
Result: Significantly suppressed tumor growth.
Peso molecular

128.17

Fòrmula

C3H5KO3
No. CAS
Appearance

Liquid

Color

Colorless to light yellow

SMILES

OC(C([O-])=O)C.[K+]
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad
In Vitro: 

H2O : 50 mg/mL (390.11 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.8021 mL 39.0107 mL 78.0214 mL
5 mM 1.5604 mL 7.8021 mL 15.6043 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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