GA-Lectin-95-12 | Lectin Array 95 Clinisciences

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Figure adapted from Kvist et al., Diagnostics, 2023.

Lectin Array 95

RayBio^® Lectin Array 95. Detects glycan profiles using 95 lectins. Suitable for all liquid sample types.

Product Description

Specifications

Size	12 Sample Kit, 24 Sample Kit, 48 Sample Kit
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	95
Solid Support	Glass Slide
Design Principle	Sandwich-based, Label-based
Method Of Detection	Fluorescence Laser Scanner
Research Area	Post-Translational Modifications, Glycosylation
Shipping Type	Blue ice
Storage	-20°C

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 12 samples on each slide
Suitable for high-throughput assays

Introduction

Lectins are glycan-binding proteins that have been purified from trees, beans, and some fruits. They are highly specific for a given glycan based on their sequence and the different sugar unit structures the glycan contains. The Lectin Array 95 consists of a standard glass slide spotted with 12 identical lectin arrays (1 per well), each containing 95 unique lectins spotted in duplicate, plus positive controls. The slide comes with a 16-well removable gasket which allows for the processing of 12 samples using one slide. Four slides can be nested into a tray, which matches a standard microplate and allows for the automated robotic high-throughput processing of 48 arrays simultaneously. The RayBio^® Lectin Array 95 array provides a powerful new tool for glycosylation determination, drug discovery, and biomarker development, all while requiring limited sample volumes.

Lectin Names

AAA, AAL, ABA, ABL, ACG, ACL, AMA, ASA, BanLec, BC2L-A, BC2LCN, BPA, CA, CAA, Calsepa, CGL2, CNL, Con A, CPA, CSA, DBA, Discoidin I, Discoidin II, DSA, ECA, EEL, F17AG, Gal1, Gal1-S, Gal2, Gal3, Gal3C-S, Gal7-S, Gal9, GHA, GNA, GRFT, GS-I, GS-II, HAA, HHA, HMA, IRA, Jacalin, LAL, LBA, LCA, LEA, Lentil, Lotus, LPA, LSL-N, MAA, Malectin, MNA-G, MNA-M, MOA, MPL, NPA, Orysata, PA-IIL, PA-IL, PALa, PHA-E, PHA-L, PHA-P, PNA, PPL, PSA, PSL1a, PTL-1, PTL-2, PWA, RCA-120, RCA-60, RPA, RS-Fuc, SAMB, SBA, SHA, SJA, SNA-I, SNA-II, SSA, STL, TL, UDA, UEA-I, UEA-II, VFA, VRA, VVA, VVA-M, WFA, WGA

Typical Data

GA-Lectin-95 was used to profile lectin-binding glycan patterns in saliva from pediatric TBI patients and healthy controls, with a few representative lectins shown to illustrate glycan differences associated with brain injury. Figure adapted from Kvist et al., Diagnostics, 2023.

GA-Lectin-95 was used to profile plasma glycan-binding patterns in early-stage non-small cell lung cancer patients and healthy controls, with heat map analysis revealing distinct glycosylation signatures associated with disease status. Figure adapted from Li et al., Frontiers in Oncology, 2023.

GA-Lectin-95 was used to profile global glycosylation changes in cervical cancer cells expressing HPV16 E6, with representative lectin array images showing increased binding to α2,6-sialylated glycans compared to control cells. Figure adapted from Wang et al., Frontiers in Oncology, 2021.

Application Data/Notes

Application 1 - Detection of Glycans on a Purified Protein

See Image/s

In this application, the RayBio Lectin Array 95 was used to detect specific glycosylations of purified Horseradish Peroxidase (HRP). Lectins BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa showed strong signals after incubation with 3.3 ug/mL Biotin-HRP followed by detection with streptavidin-fluorescence-dye (Figures A, B and C). The fluorescence signals from BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa were blocked in a concentration-dependent manner by HRP itself (Figures A and C), indicating that the signals were generated by lectin-HRP binding. These eight lectins are known to exhibit specific binding to mannose, which indicates that HRP contains mannose. After adding increasing amounts of mannose, the signals from BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa were reduced (Figures A and B).The reduction in signals from increasing concentrations of mannose confirms that HRP protein contains mannose in its glycocalyx. Additionally, the two lectins AAL and RS-FUC (fucose binding specificity) also showed strong interaction with HRP, which indicates the fucosylation of HRP. Overall, the results of the Lectin Array 95 were consistent with published literature regarding HRP glycosylation.

Application 2 - Profiling of a Serum Sample

See Image/s

Using the lectin 95 array, we can discover the different glycoprotein profiles of serum samples, cell lysates, or purified glycoproteins. The images above show the profiles of the glycans from different types of samples including human serum, recombinant glycoproteins human HE4 and AFP, mouse TFF2, purified human IgG, and bacterial DE3 cell lysates detected by Biotin labeling and fluorescent dye-streptavidin.

Suggested Applications

Identify and profile the glycans in their samples
Determine whether their biomarker of interest has glycan moieties
Find specific glycan binding ligands in biological samples

Other Applications

Quantitative analysis of lectin-glycoprotein interactions. Example: a concentration series of glycoproteins detected with the lectin array could reveal concentration dependent effects of lectin-glycan binding.

Determine the profile of bacterial cell-surface glycans. Example: Cell lysate from bacteria can be Biotinylated and hybridized to the lectin array. Analysis of the binding pattern and correlation with the known carbohydrate-binding specificities of the lectins can determine the glycans on the cell membrane.

Kit Components

Dialysis Vials
Labeling Reagent
Labeling Buffer
Stop Solution
Lectin Array Glass Slide Assembly
Sample Diluent
20X Wash Buffer I
20X Wash Buffer II
Cy3 equivalent dye-conjugated Streptavidin
Slide Washer/Dryer
Adhesive device sealer
Floating Dialysis Rack
Manual

Additional Materials Required

Detection antibodies of interest (For sandwich-based method only)
Orbital shaker
Laser scanner for fluorescence detection
Aluminum foil
1.5ml Polypropylene microcentrifuge tubes
KCl, NaCl, KH₂PO₄ and Na₂HPO₄ (For label-based method only)
Plastic or glass containers, beaker, stir plate and stir bar
Pipettors, pipette tips, ddH₂O and other common lab consumables

Assay Procedure Summary

Dry the glass slide
Block array surfaces
Incubate samples (samples need to be biotinylated for the label-based approach)
For the the sandwich-based principle, incubate with a detection antibody cocktail
For the label-based principle, incubate the labeled-streptavidin.
Incubate with Cy3 Equivalent Dye-Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the Raybiotech Lectin Array 95 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.

documents

Datasheet (GA-Lectin-95.pdf)

SDS (GA-Lectin-95.pdf)

GAL (GAL-GA-Lectin-95.gal)

Excel (GA-LECTIN-95_Analysis_Tool.xlsx)

citations

Identification of SARS-CoV-2-binding lectins on a commercial lectin array
Species:
Other
Sample Type:
Purified / Recombinant Protein (recombinant spike glycoprotein, cultured SARS-CoV-2 virus)
Year:
2025
Journal Name:
Scientific Reports
PubMed ID:
40593795
Comparative phenotyping of surface markers and glycans in murine and human platelet-derived extracellular vesicles
Species:
Human
Sample Type:
Tissue Lysate / Homogenate (l-PEV lysates)
Year:
2026
Journal Name:
Research and Practice in Thrombosis and Haemostasis
PubMed ID:
42011386
SENSING SWEET SPOTS: A comprehensive study of SARS-CoV-2-binding lectins
Species:
Other
Sample Type:
Purified / Recombinant Protein (recombinant spike glycoprotein, cultured SARS-CoV-2 virus)
Year:
2024
Journal Name:
Research Square
Glycoprofiling of early non-small cell lung cancer using lectin microarray technology
Species:
Human
Sample Type:
Plasma
Year:
2023
Journal Name:
Oncologie
Cell surface carbohydrates of symbiotic dinoflagellates and their role in the establishment of cnidarian-dinoflagellate symbiosis
Species:
Other
Sample Type:
Other / Unspecified (cell-surface glycoproteins)
Year:
2022
Journal Name:
The ISME journal
PubMed ID:
34285364
Glycans as Potential Diagnostic Markers of Traumatic Brain Injury in Children
Species:
Human
Sample Type:
urine, saliva
Year:
2023
Journal Name:
Diagnostics
Dectin-2 mediates phagocytosis of Lactobacillus paracasei KW3110 and IL-10 production by macrophages
Species:
Other
Sample Type:
Other / Unspecified (LAB species)
Year:
2021
Journal Name:
Scientific Reports
PubMed ID:
34489491
High-density lectin microarray uncovers stage-specific plasma glycoprofiling signatures for non-muscle-invasive and muscle-invasive bladder cancer
Species:
Human
Sample Type:
Plasma (22 NMIBC, 10 MIBC, 16 controls)
Year:
2026
Journal Name:
International Urology and Nephrology
PubMed ID:
40783897
High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells
Year:
2021
Journal Name:
Frontiers in oncology
PubMed ID:
34745942
Glycoprofiling of Early Gastric Cancer Using Lectin Microarray Technology
Year:
2018
Journal Name:
Clinical Laboratory
PubMed ID:
29479898

Lectin Array 95

Referencia GA-Lectin-95-12

Marca : RayBiotech

Lectin Array 95