Evercyte’s human myoblast cell line LHCN-M2 can be grown without limitations while maintaining expression of cell type specific markers and function. Therefore, these cells are useful for screening substances influencing muscle contraction, to study myotoxicity or muscle damage. Additionally, the cell line is the perfect starting material for genetic engineering to create important disease models such as Duchenne muscular dystrophy or Facioscapulohumeral muscular dystrophy.
General information
Cat#: CkHT-040-231-2
Morphology and marker expression
LHCN-M2 cells are characterized by the typical, myoblast morphology.
The cells homogenously express CD56 as demonstrated by immunofluorescence staining.
Differentiation
Differentiation of LHCN-M2 cells leads to increased expression of typical myoblast markers desmin, MF-20 and Pax7 concomitant with fusion of cells and formation of myotubes.
FAQs
In vitro propagation
MyoUp ready-to use medium (Cat# MHT-040)
DMEM (Gibco, Cat# 61965-026)/M199 (Gibco, Cat# 31150022) 4+1
15 % Fetal bovine serum (PAN Biotech, Cat# P30-3031)
20 mM Hepes (Sigma-Aldrich, Cat# H0887)
3 µg/ml Zinc sulfate (Sigma-Aldrich, Cat# Z0251)
1.4 µg/ml Vitamin B12 (Sigma-Aldrich, Cat# V2876)
0.055 µg/ml Dexamethasone (Sigma-Aldrich, Cat# D4902)
2.5 ng/ml HGF (Merck Millipore, Cat# GF116)
5 ng/ml bFGF (Enantis, Cat# FGF-STAB)
Additional material & reagents
0,1 % Gelatin from porcine skin – Type A (Sigma-Aldrich, Cat# G1890, prepare in sterile water)
Phosphate buffered saline (PBS) (Gibco, Cat# 14190-144)
0,05 % Trypsin-EDTA (Gibco, Cat#25300-054)
Passaging of cells
The new culture flasks have to be pre-coated with porcine gelatin. Therefore, the culture flasks are treated with Gelatin solution (80 µl/cm²) at 37°C for at least 4 hours (up to one week).
Cryopreservation
Freezing medium
DMEM (Gibco, Cat # 61965-026) / M199 (Gibco, Cat# 31150022, 4+1
15 % Fetal bovine serum (PAN Biotech, Cat# P30-3031)
10 % DMSO (Sigma-Aldrich, Cat# D2650)
Additional material & reagents
0,1 % Gelatin from porcine skin – Type A (Sigma-Aldrich, Cat# G1890, prepare in sterile water)
Phosphate buffered saline (PBS) (Gibco, Cat# 14190-144)
Related Products
MyoUp cell culture medium for growth of LHCN-M2 cells
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