Snippet: 6 overexpression, the STAT6 cDNA was cloned into the PGMLV-CMV-3×Flag-PGK-Puro vector (Genomeditech, Shanghai, China) and transfected into target cells using the MegaTran V.2.0 transfection reagent (Origene, USA). Stable cell populations expressing STAT6 were selected by adding puromycin (5 µg/mL) after 72 hours. Western blotting and quantitative PCR analysis were employed to confirm the eff

Snippet: been identified, wherein its hyper-phosphorylation, premature nuclear retention, enhanced DNA-binding affinity, and subsequent upregulation of target genes are known to drive several hallmarks of tumorigenesis, including metastasis, immune evasion, and drug resistance. Due to its regulatory role in cell proliferation and differentiation, mutations in STAT3 have been associated with poor prognosis;...

Snippet: …Consequently, WNT ligand-receptor interaction is extremely promiscu;- Background The WNT signal pathway has myriad family members, which are broadly involved in embryonic development and human cancer. Over-activation of WNT-β- Save;s, 1.5 µg Δ8.9 plasmid and 1 µg VSV-G plasmid using Megatran1.0 (OriGene, #TT210002) according to the manufacturer’s instructions