Mix-n-Stain™ Maxi FITC Antibody Labeling Kit, 1x1mg labeling
Referencia 92411
embalaje : 1kit
Marca : Biotium
Mix-n-Stain™ Maxi Antibody Labeling Kits, 1 mg Labeling
The simplicity and convenience of Mix-n-Stain™ labeling, for conjugation of 1 mg IgG with our CF® Dyes or other dyes.
Mouse anti-transferrin receptor antibody from (endosome and plasma membrane marker) was labeled using Lightning-Link® Rapid DyLight® Conjugation Kits from Novus Biologicals (left) or Mix-n-Stain™ CF® Dye Antibody Labeling Kits (right) according to manufacturers’ instructions. The CF® dye conjugates show higher signal and more specific staining compared the DyLight®conjugates when imaged using the same settings, due to the superior brightness of CF® dyes. The insets show the same field of view imaged with a higher gain setting.
Monoclonal mouse anti-tubulin clone DM1A in ascites or purified in PBS with 1% BSA was labeled with CF®488A Mix-n-Stain using the modified protocol for total protein labeling. The Mix-n-Stain antibodies performed comparably in immunofluorescence staining of HeLa cells as unlabeled antibody (in ascites) detected with secondary antibody.
Comparison of Mix-n-Stain™ CF®647 labeling of anti-TfR antibody (endosome and cell surface marker) in HeLa cells without added protein compared to 1% BSA or 1% gelatin (10-fold excess) using the modified protocol for labeling in the presence of stabilizer protein. Labeling using the modified protocol gives comparable results as the standard protocol, with bright signal and minimal increase in non-specific background.
Mix-n-Stain™ labeling can be performed on unpurified antibodies in ascites fluid. Top: HeLa cells stained with anti-tubulin antibody in ascited, detected with CF®488A secondary antibody (left) or directly labeled using CF®488A Mix-n-Stain™ (right). Bottom: rat optic nerve cryosections stained with anti-GFAP (glial marker) detected with CF®568 secondary antibody or directly labeled with Mix-n-Stain™ CF®568. antibodies in Mix-n-Stain™ labeled ascites show comparable specificity to unlabeled antibodies detected using secondary antibody.
Rat testis with mouse anti-tubulin and CF®488A goat anti-mouse (min x rat) (green), CF®555 Mix-n-Stain labeled mouse anti-ZO1 (tight junctions, red) and CF®640R phalloidin (cyan).
HeLa cells stained with CF640R Mix-n-Stain labeled mouse anti-tubulin antibody (red). Actin filaments are stained with CF488A phalloidin (green). Mounted in Everbrite Mounting Medium with DAPI (nuclei, blue).Product Description
Mix-n-Stain™ Maxi Antibody Labeling Kits allow you to rapidly label 1 mg of IgG with one of our next-generation CF® Dyes or other label.
- Rapid, simple, covalent antibody conjugation
- No purification required after labeling
- Reaction takes only 30 minutes, with minimal hands-on time
- Wide selection of our next-gen CF® Dyes and other dyes
- One labeling reaction of 1 mg IgG per kit
Kit Components
- Reaction Buffer
- Lyophilized Reactive Dye
- Quenching Buffer
Download the Product Information Sheet (PI) for detailed instructions.
Unrivaled Convenience
Mix-n-Stain™ Maxi Antibody Labeling Kits allow you to rapidly label 1 mg of IgG with one of our next-generation CF® Dyes or other label. Simply mix your antibody with the reaction buffer and optimally formulated dye provided, followed by a 30 minute incubation and addition of the provided stop buffer. Any remaining free dye or other label is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. Note the labeling protocol and buffer requirements for the Maxi kits differ from our original micro-scale Mix-n-Stain™ kits, see the Reaction Compatibility Chart below.
After the reaction, the antibody will be labeled with an average of 4-6 dye molecules per antibody molecule. Mix-n-Stain™ labeling is covalent, so labeled antibodies can be used for multiplex staining without transfer of dyes/labels between antibodies.
Superior CF® Dyes
These kits feature a choice of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. We also offer kits for labeling with Cyanine 555, Cyanine 647, or fluorescein/FITC. See Ordering Information below.
Choose the Right Kit for Your Application
These kits are optimized for a single labeling reaction with 1 mg of IgG. Mix-n-Stain™ kits are not designed to be used for multiple labeling reactions. For smaller-scale conjugation, see our original Mix‑n‑Stain™ CF® Dye Antibody Labeling Kits; original Mix-n-Stain™ kits also have greater tolerance for antibody buffer additives, and provide special protocols for labeling antibodies in buffer with BSA or in ascites fluid. The kits are not recommended for labeling IgM antibodies, which may denature under the reaction conditions.
Mix-n-Stain™ kits can be used to label other proteins, however, the degree of labeling (DOL, or number of dye molecules per antibody) may not be optimal, and the kits are not designed to allow for DOL measurement after labeling. For performing reactions to optimize DOL for your antibody or application, we recommend using our CF® Dye SE Protein Labeling Kits. The kits include everything you need to perform controlled conjugation reactions and post-labeling purification, as well as detailed instructions and correction factors for determining DOL for CF® Dyes.
Learn about all of our Mix-n-Stain™ and protein labeling options, or see our Frequently Asked Questions (FAQs) for more information.
Reaction Compatibility for Mix-n-Stain™ Maxi Antibody Labeling Kits, 1 mg Labeling
| Component | Compatibility |
|---|---|
| Sodium azide | Compatible |
| Glycerol ≤ 10%: | Compatible |
| Glycerol > 10%: | Compatible after ultrafiltration* |
| Tris ≤20 mM | Compatible |
| Tris >20 mM | Compatible after ultrafiltration* |
| Glycine | Compatible after ultrafiltration* |
| BSA or gelatin | Not compatible; purify antibody** |
| Serum, cell culture supernatant, or ascites fluid | Not compatible; purify antibody** |
** Protocol/reagents for antibody purification not provided.
Mix-n-Stain™ Maxi Ordering Information
| Conjugation | Ex/Em | Labeling size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®350 | 347/448 nm | 1 mg | 92420 | CF®350 Features |
| CF®405S | 404/431 nm | 1 mg | 92421 | CF®405S Features |
| CF®405M | 408/452 nm | 1 mg | 92404 | CF®405M Features |
| CF®405L | 395/545 nm | 1 mg | 92454 | CF®405L Features |
| CF®488A | 490/515 nm | 1 mg | 92405 | CF®488A Features |
| CF®555 | 555/565 nm | 1 mg | 92406 | CF®555 Features |
| CF®568 | 562/583 nm | 1 mg | 92407 | CF®568 Features |
| CF®594 | 593/614 nm | 1 mg | 92408 | CF®594 Features |
| CF®633 | 630/650 nm | 1 mg | 92409 | CF®633 Features |
| CF®647 | 650/665 nm | 1 mg | 92410 | CF®647 Features |
| CF®680 | 681/698 nm | 1 mg | 92422 | CF®680 Features |
| CF®750 | 755/777 nm | 1 mg | 92423 | CF®750 Features |
| CF®770 | 770/797 nm | 1 mg | 92424 | CF®770 Features |
| Fluorescein (FITC) | 494/518 nm | 1 mg | 92411 | |
| Cyanine 555 | 555/565 nm | 1 mg | 92415 | |
| Cyanine 647 | 650/665 nm | 1 mg | 92419 |
Antibody & Protein Labeling Kits
| Product Name | Label Options | Labeling Scale | Labeling Time | Features |
|---|---|---|---|---|
| Mix-n-Stain™ CF® Dye Antibody Labeling Kits | CF® Dyes | ≤5-20 ug IgG 20-50 ug IgG 50-100 ug IgG | ~ 15 min. | • Rapid, simple labeling • No purification • Tolerates BSA & other additives • Dyes for super-resolution, spectral flow, & NIR detection |
| Mix-n-Stain™ FITC Antibody Labeling Kits | FITC | |||
| Mix-n-Stain™ Cyanine Dye Antibody Labeling Kits | Cyanine 555 Cyanine 647 |
|||
| Mix-n-Stain™ Biotin Antibody Labeling Kits | Biotin | |||
| Mix-n-Stain™ Digoxigenin Antibody Labeling Kits | Digoxygenin (DIG) | |||
| Mix-n-Stain™ DNP Antibody Labeling Kits | DNP | |||
| Mix-n-Stain™ Maxi Antibody Labeling Kits | CF® Dyes Cyanine Dyes | 1 mg IgG | ~ 30 min. | |
| Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits | CF® Dyes FITC | 25 ug IgM 100 ug IgM | 15-30 min. | • Rapid, simple labeling • No purification |
| Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits | CF® Dyes | 50 ug IgG | ~ 30 min. | • Optimized to provide low 1-2.5 DOL • No purification |
| Mix-n-Stain™ Nanobody Labeling Kits | CF® Dyes | 5-20 ug Nanobody® 20-50 ug Nanobody® | ~ 30 min. | • Optimized for Nanobodies® • Tolerates BSA & other additives |
| Mix-n-Stain™ Nanobody Thiol Labeling Kits | CF® Dyes | ~ 2 hours | • Optimized for Nanobodies® with single exposed cysteine residue | |
| Mix-n-Stain™ Small Ligand Labeling Kits | CF® Dyes | 0.1 umol small ligand | ~ 30 min. | • Label SNAP®, CLIP™, HaloTag® & other small ligands • Dye options for surface or intracellular targets |
| VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging | Near-IR CF® Dyes | 3 x 1 mg IgG | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification • Sterile syringes, filters and storage vials for in vivo use |
| CF® Dye & Biotin SE Protein Labeling Kits | CF® Dyes Biotin | 3 x 1 mg protein | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification |
Technology
Product Attributes
Labeling scale
Purification method
Documents, Protocols, SDS and COA
Documents
COA
Request the Certificate of AnalysisReferences
Download a list of curated CF® Dye references.
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