Mouse Brachyury protein (T) ELISA Kit
Referencia AE16202MO-480
embalaje : 5x96tests
Marca : Abebio
Reagent Preparation
Results demonstration
Assay Procedure Summary
Certificate
Product Details
| Species Reactivity | Mouse (Mus musculus) |
| UniProt | P20293 |
| Abbreviation | T |
| Alternative Names | RP11-459F1.1; MGC104817; TFT; OTTHUMP00000017588|T brachyury homolog|T brachyury-like|transcription factor T |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1 - 2 0 0 μ L |
| Detection Wavelengt | 450 nm |
Test principle
This assay employs a two-site sandwich ELISA to quantitate T in samples. An antibody specific for T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any T present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview
Components
Reagents | Quantity | Reagents | Quantity |
Assay plate (96 Wells) |