Background: Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for highenergy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and pcreatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from pcreatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.

Principle of the Assay: Creatinine is catalyzed by creatininase to form creatine, and creatine is catalyzed by creatinase to form sarcosine and urea, then sarcosine catalyzed by sarcosine oxidase to form glycine, formaldehyde and hydrogen peroxide. Hydrogen peroxide and chromogenic agent react under the catalysis of peroxidase to produce pink compound The creatinine content was calculated by ready the OD value at 546 nm(dominant wavelength)/700 nm (Deputy wavelength).

Small volumes of Creatinine assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS763433 is an assay kit targeting Creatinine. Also known as Mouse Creatinine (Colorimetric) Assay Kit. Reactive in Mouse.