Mouse FPR2 (Formyl Peptide Receptor 2) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	Fpr2
Gene ID:	14289
Uniprot ID:	FPR2_MOUSE
Specificity:	This kit recognizes Mouse FPR2 in samples. No significant cross-reactivity or interference between Mouse FPR2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Primarily expressed in neutrophils. Not detected in vomeronasal neurons.
Function	High affinity receptor for N-formyl-methionyl peptides (FMLP), which are powerful neutrophil chemotactic factors. Stimulates chemotaxis in immune cells to site of infection or tissue damage upon recognition of several ligands, such as FMLP, or ligand involved in cell damage, disease or inflammation. Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation.
Protein Name	Formyl Peptide Receptor 2 Formylpeptide Receptor-Related Sequence 2 Lipoxin A4 Receptor-Like Protein N-Formylpeptide Receptor-Like 2
Database Links	Reactome: R-MMU-416476 Reactome: -MMU-418594 Reactome: -MMU-444473 Reactome: -MMU-6798695
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Associates With Amyloid-Beta Protein 42 Product Of App At The Cell Surface And The Complex Is Then Rapidly Internalized
Alternative CLIA Names	Formyl Peptide Receptor 2 CLIA kit Formylpeptide Receptor-Related Sequence 2 CLIA kit Lipoxin A4 Receptor-Like Protein CLIA kit N-Formylpeptide Receptor-Like 2 CLIA kit Fpr2 CLIA kit Fpr-rs2 CLIA kit
Specificity	This kit recognizes Mouse FPR2 in samples. No significant cross-reactivity or interference between Mouse FPR2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-101	92
EDTA plasma(n=8)	92-104	98
Cell culture media(n=8)	87-99	94

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	40.32	72.77	367.79	37.73	76.79	402.90
Standard deviation	3.92	6.64	30.05	4.83	8.35	26.75
CV (%)	9.72	9.12	8.17	12.80	10.87	6.64

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance