Mouse IgM Kappa (κ) isotype Control
Referencia ASR2274
embalaje : 1mg
Marca : Abcepta
Mouse IgM Kappa (κ) isotype Control
Monoclonal MMK IgM , Unconjugated
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SDS-PAGE of Mouse IgM Kappa isotype control . Lane 1: 5 µL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab) Fragment . Lane 5: Mouse IgM Kappa isotype control . Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM ? at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-PAGE of Mouse IgM Kappa isotype control . Lane 1: 5 µL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab) Fragment . Lane 5: Mouse IgM Kappa isotype control . Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM ? at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
| Description | MOUSE IgM Kappa (κ) isotype control |
|---|---|
| Conjugate | Unconjugated |
| Clonality | Monoclonal |
Application 
| FC |
| Application Note | FlowCytometry 1:1000-1:5000 |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgM |
| Buffer | 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 |
| Species of Origin | Mouse |
| Preservative | 0.1% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Purity | Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Light and heavy chain composition confirmed by Elisa. |
| Storage Condition | Store vial at 4° C prior to opening. Mouse IgM Kappa isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
- Secondary Antibodies
- Peroxidase (Horseradish)
- Unconjugated