RayBio® Mouse MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Mouse MCP-1 ELISA Kit
Mouse MCP-1 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 3 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 - 10 fold |
| Accession Number | P10148 |
| Gene Id | 20296 |
| Gene Symbols | CCL2, SCYA2, MCP1, JE |
| Protein Name / Synonyms | C-C motif chemokine 2 (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Platelet-derived growth factor-inducible protein JE) (Small-inducible cytokine A2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
NexaTag® Mouse MCP-1 (CCL2) qIPCR ELISA Kit
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse MCP-1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MCP-1 (CCL2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Adipose-specific deletion of the cation channel TRPM7 inhibits TAK1 kinase-dependent inflammation and obesity in male mice
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Nature Communications
Anti-Inflammatory Resveratrol Protects Mice From Early Mortality After Haematopoietic Stem Cell Transplantation
- Species:
- Mouse
- Sample Type:
- Serum (HSCT mice treated with resveratrol)
- Year:
- 2025
- Journal Name:
- Journal of Cellular and Molecular Medicine
- PubMed ID:
- 39900564
Maternal polysorbate 80 exposure causes intestinal ILCs and CD4+ T cell developmental abnormalities in mouse offspring
- Species:
- Mouse
- Sample Type:
- fecal
- Year:
- 2023
- Journal Name:
- Environmental Pollution
Phloretin and phlorizin mitigates inflammatory stress and alleviate adipose and hepatic insulin resistance by abrogating PPARγ S273-Cdk5 interaction in type 2 diabetic mice
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- LIFE SCIENCES
Inhibition of CCL2 by bindarit alleviates diabetes-associated periodontitis by suppressing inflammatory monocyte infiltration and altering macrophage properties
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant; Serum; Cell Lysate; Tissue Lysate / Homogenate (gingival tissue)
- Year:
- 2021
- Journal Name:
- Cellular & Molecular Immunology
- PubMed ID:
- 32678310
CCL2/CCR2 Signalling in Mesenchymal Stem/Progenitor Cell Recruitment and Fracture Healing in Mice
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (macrophage conditioned media)
- Year:
- 2024
- Journal Name:
- Journal of Cellular and Molecular Medicine
- PubMed ID:
- 39721002
EMP3 mediates glioblastoma-associated macrophage infiltration to drive T cell exclusion
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (culture supernatants of GL261 cells)
- Year:
- 2021
- Journal Name:
- Journal of Experimental & Clinical Cancer Research
- PubMed ID:
- 33964937
Elevated Kallikrein-binding protein in diabetes impairs wound healing through inducing macrophage M1 polarization
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (mouse plasma or cellular supernatants)
- Year:
- 2019
- Journal Name:
- Cell Communication and Signaling
- PubMed ID:
- 31182110
Tissue specificity of senescent cell accumulation during physiologic and accelerated aging of mice
- Species:
- Mouse
- Sample Type:
- Serum (aged WT and Ercc1−/∆ mice); Tissue Lysate / Homogenate
- Year:
- 2020
- Journal Name:
- Aging Cell
- PubMed ID:
- 31981461
Methanolic fruit extract of Myrica nagi protects the hypothalamus and attenuates inflammation associated with gold thioglucose- and high-fat diet-induced obesity via various adipokines
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Journal of Ayurveda and integrative medicine
