- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse OxLDL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled OxLDL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
Mouse oxidized low density lipoprotein,OxLDL ELISA Kit
Referencia CSB-E07933m-96T
embalaje : 96T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
oxidized lowdensity lipoprotein,OxLDL
Abbreviation
OxLDL
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.45 nmol/mL-30 nmol/mL
Sensitivity
0.11 nmol/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-87 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 83-96 | |||||
Recovery
| The recovery of mouse OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Mouse OxLDL ELISA Kit was designed for the quantitative measurement of Mouse OxLDL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 nmol/mL-30 nmol/mL and the sensitivity is 0.11 nmol/mL .
Citations
- XJ Lin, M Yao, WY Lin, Q Yuan,Molecular Medicine Reports,2026
- S Zhang, M Yan, P Liang, Y Zhang, J Liu,Diabetes, Metabolic Syndrome and Obesity,2025
- Z Zhang, M Chen, Y Xu, Z Wang, Z Liu, C He,Theranostics,2025
- S Zhang, J Liu, X Ouyang, P Lyu, Y Wang,Journal of Dental Sciences,2024
- Z Wang,Molecular medicine,2023
- C Cheng,Signal transduction and targeted therapy,2023
- MC Morrison,FASEB journal,2023
- S Panyod,NPJ science of food,2023
- DA Alshammaa,Biomedical and Pharmacology Journal,2022
- Ginger Essential Oil and Citral Ameliorate Atherosclerosis Via Modulating TMAO and Gut Microbiota in ApoE −/− Mice Fed on Gubra Amylin NASH Diet with ʟ-Carnitine LY Sheen,researchsquare,2022
Customer Reviews and Q&A
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■ Q&A
Q:
I would like to know which portion of OXLDL is recognized by your mouse oxLDL kit cat# CSB-E07933m.
A:
Thanks for your inquiry.
The standard of this kit is OXLDL prepared by the oxidation of cupric sulfate.
IIt can detect endogenous OXLDL.
At the same time, the OXLDL prepared by different methods can be detected as long as the corresponding recognition sites/regions are available.
Sorry we are not clear about the specific recognition area. If you want to detect other methods to...
The standard of this kit is OXLDL prepared by the oxidation of cupric sulfate.
IIt can detect endogenous OXLDL.
At the same time, the OXLDL prepared by different methods can be detected as long as the corresponding recognition sites/regions are available.
Sorry we are not clear about the specific recognition area. If you want to detect other methods to...