Nucleolin (NCL) Mouse Monoclonal Antibody [Clone ID: 4E2]
Specifications
Product Data | |
Clone Name | 4E2 |
Applications | FC, IF, IHC, WB |
Recommended Dilution | Western blot: 1 µg/ml for chemiluminescence detection system. Immunohistochemistry (paraffin sections): 1-10 µg/ml. Immunocytochemistry: 1-10 µg/ml. Flow cytometry: 5-10 µg/ml (final concenctration). |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human nucleolin from Raji cell extract |
Specificity | This antibody detects nucleolin. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservatives are contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Database Link | |
Background | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. |
Synonyms | NCL |
Note | This product was originally produced by MBL International. Protocol: SDS - PAGE & Western Blotting Immunohistochemical staining for paraffin - embedded sections : SAB method Immunocytochemistry Flow cytometric analysis for cells We usually use Fisher tubes or equivalents as reaction tubes for all step s described below. |
Reference Data |
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