Currency:  Select mL) 01-0005 05-100-1A 500 mL (select above) .00 € 353.00 NutriStem™ hPSC XF Culture Medium (100 mL) 01-0005-100 05-100-1B 100 mL (select above) .00 £ Ask us € Ask us

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Notice to clinical users: A Drug Master File for NutriStem medium has been filed with the US FDA. Please contact us for more information.

NutriStem hPSC XF Medium (formerly called NutriStem XF/FF Culture Medium) is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo. NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications.

• Xeno-free, feeder-free conditions contain no animal components
• Easy, one-step transition from feeder-dependent culture, no adaptation required
• Maintains pluripotency, normal morphology, karyotype, and differentiation potential of human ES and iPS cells over long term culture
• Robust attachment and high cloning efficiency from single cells

• Amenable to a weekend-free culture  

Morphology of Human H1 ES cells Grown in NutriStem hPSC XF Medium for 20 passages.

Expression of Pluripotency Markers in hES Cells Cultured in NutriStem hPSC XF medium.

Proliferation Growth Rate of Human H1 ES cells Cultured in NutriStem hPSC XF Medium versus Competitor Medium.

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The Sartorius company name and logo are the property of Sartorius GmbH, Germany.

Product Name: NutriStem hPSC XF Medium

Catalog Number

• 01-0005 (Sartorius Cat # 05-100-1A): 500 mL
• 01-0005-100 (Sartorius Cat # 05-100-1B): 100 mL

Storage and Stability: Store at −20°C. This product is stable for a minimum of 3 months when stored as directed. See product label for lot-specific expiration date

Quality Control: NutriStem Medium tested negative for mycoplasma. Human pluripotent stem cells expressed the pluripotency markers SSEA-4, TRA-1-60, and TRA-1-81 after three continuous passages in NutriStem Medium

Sterility: Sterile

Notice To Purchaser: REPROCELL is an authorized global distributor of select cell culture products from Sartorius AG (Germany).

Manufacturer:  Biological Industries, a subsidiary of Sartorius GmbH (Germany)

Related Products:

• StemRNA 3rd Gen Reprogramming Kit
• NutriFreez D10 Freezing Medium 

Specification Sheets:

• 01-0005 Specifications Sheet

Safety Data Sheets:

• 01-0005 and 01-0005-100 Safety Data Sheet

Product Flyers:

• NutriStem™ hPSC XF Culture Medium

Protocols:

• Protocol: Nutristem hPSC XF Culture Medium

Application Notes:

• NutriStem: a Defined, Low-Growth Factor, Xeno-Free Medium for the Long Term Culture of Undifferentiated Human ES Cells

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• Devito LG; Lionello VM; Muntoni F; Tedesco FS; Healy L. Generation of an MTM1-mutant iPSC line (CRICKi008-A) from an individual with X-linked myotubular myopathy (XLMTM). Stem Cell Res 69:102079 (2023).
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• Devito LG; Cooper F; D'Angelo I; Smith J; Healy L. Generation of FOUR iPSC lines (CRICKi004-A; CRICKi005-A; CRICKi006-A, CRICKi007-A) from Spinal muscle atrophy patients with lower extremity dominant (SMALED) phenotype. Stem Cell Res 65:102954 (2022).
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• Kathuria A; Lopez-Lengowski K; Vater M; McPhie D; Cohen BM Karmacharya R. Transcriptome analysis and functional characterization of cerebral organoids in bipolar disorder. Genome Medicine 12:34 (2020).
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• Sacco AM; Belviso I; Romano V; Carfora A; Schonauer F; Nurzynska D; Montagnani S; Di Meglio F; Castaldo C. Diversity of dermal fibroblasts as major determinant of variability in cell reprogramming. J Cell Mol Med :1-13; https://doi.org/10.1111/jcmm.14316 (2019).
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• Supharattanasitthi W; Carlsson E; Sharif U; Paraoan L. CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection. Sci Rep 9:174 (2019).
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• Ma L; Hu J; Cao Y; Xie Y; Wang H; Fan Z; Zhang C; Wang J; Wu C-T; Wang S;. Maintained Properties of Aged Dental Pulp Stem Cells for Superior Periodontal Tissue Regeneration. Aging and Disease :https://doi.org/10.14336/AD.2018.0729 (2018).
• Goa X; Yourick JJ; Sprando RL. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel. Stem Cell Researchhttps://doi.org/10.1016/j.scr.2018.07.013: (2018)
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