Phosphate, PBlue, BioAssay™ Kit

Intended Use:
Phosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate.
Lipase Assays: liberation of phosphate from phospholipids
Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
Quantitation of Phosphate: in phospholipids, proteins and DNAs, etc.
Drug Discovery: high-throughput screen for phosphatase inhibitors.

Sensitivity:
Detection of as little of 20pmoles of phosphate

Range:
0.4-50uM

Test Principle:
The PBlue Phosphate BioAssay™ Kit is based on a proprietary formulation of the malachite green dye. The PBlue reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660nm) or on a plate reader. The non-radioactive colorimetric assay kits have been
optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high throughput screening of enzyme inhibitors.

Kit Components:
Reagent, 1x50ml
Standard, 1mM phosphate, 1x1ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.