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> Phospholipase C Protein, Clostridium perfringens, Recombinant (His)

Phospholipase C Protein, Clostridium perfringens, Recombinant (His)

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Referencia NB-64-49099-200ug

embalaje : 200ug

Marca : Neo Biotech


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Phospholipase C Protein, Clostridium perfringens, Recombinant (His)

(Synonyms: PLC, Phospholipase C, Phosphatidylcholine cholinephosphohydrolase, Lecithinase, Hemolysin, cpa, Alpha-toxin) Copy Product Info

Synonyms: PLC, Phospholipase C, Phosphatidylcholine cholinephosphohydrolase, Lecithinase, Hemolysin, cpa, Alpha-toxin

Catalog No. TMPH-00421 Copy Product Info
Phospholipase C Protein, Clostridium perfringens, Recombinant (His) is expressed in E. coli.
Other Forms of Phospholipase C Protein, Clostridium perfringens, Recombinant (His):
Phospholipase C Protein, S. aureus, Recombinant (His)TMPH-03568
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For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Phospholipase C Protein, Clostridium perfringens, Recombinant (His) is expressed in E. coli.
Species
C.perfringens
Expression System
E. coli
TagN-6xHis
Accession NumberP0C216
Amino AcidWDGKIDGTGTHAMIVTQGVSILENDLSKNEPESVRKNLEILKENMHELQLGSTYPDYDKNAYDLYQDHFWDPDTDNNFSKDNSWYLAYSIPDTGESQIRKFSALARYEWQRGNYKQATFYLGEAMHYFGDIDTPYHPANVTAVDSAGHVKFETFAEERKEQYKINTAGCKTNEDFYADILKNKDFNAWSKEYARGFAKTGKSIYYSHASMSHSWDDWDYAAKVTLANSQKGTAGYIYRFLHDVSEGNDPSVGKNVKELVAYISTSGEKDAGTDDYMYFGIKTKDGKTQEWEMDNPGNDFMTGSKDTYTFKLKDENLKIDDIQNMWIRKRKYTAFPDAYKPENIKIIANGKVVVDKDINEWISGNSTYNIK
Construction29-398 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsPLC, Phospholipase C, Phosphatidylcholine cholinephosphohydrolase, Lecithinase, Hemolysin, cpa, Alpha-toxin
Research Background
Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.
Chemical Properties
Molecular Weight48.5 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

α-toxinαtoxinPhospholipaseCa-toxin
Related Tags: Phospholipase C Protein, Clostridium perfringens, Recombinant (His) chemical structure | Phospholipase C Protein, Clostridium perfringens, Recombinant (His) molecular weight