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Rab5 Antibody

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Referencia ASM10422-STR-100

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  • ICC/IF - Rab5 Antibody ASM10422
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.
  • WB - Rab5 Antibody ASM10422
    Western blot analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
  • IHC - Rab5 Antibody ASM10422
    Immunohistochemistry analysis using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
  • ICC/IF - Rab5 Antibody ASM10422
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession Q6FI44
Other Accession NP_004153.2
Host Rabbit
Reactivity Human, Mouse, Rat, Monkey, Bovine
Clonality Polyclonal
Description Rabbit Anti-Human Rab5 Polyclonal
Target/Specificity Detects ~26kDa.
Other Names Rab 5A Antibody, RAS associated protein RAB5A Antibody, Ras related protein Rab 5 A Antibody
Immunogen Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µl/ml of SPC-168 was sufficient for detection of Rab5 in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Endosome | Early Endosome Membrane | Melanosome