Rabbit Anti-DANRE actba (Center) Polyclonal Antibody
Referencia Azb10021c
embalaje : 400ul
Marca : Abcepta
Zebrafish actba Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Zebrafish actba Antibody (Center) (Cat. #Azb10021c) western blot analysis in zebrafish muscle tissue lysates (35ug/lane).This demonstrates the Zebrafish actba antibody detected the zebrafish actba protein (arrow).
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All lanes : Anti-Zebrafish actba Antibody (Center) at 1:1000 dilution Lane 1: zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Zebrafish actba Antibody (Center) (Cat. #Azb10021c) western blot analysis in zebrafish muscle tissue lysates (35ug/lane).This demonstrates the Zebrafish actba antibody detected the zebrafish actba protein (arrow).
Application 
| WB, E |
|---|---|
| Primary Accession | Q7ZVI7 |
| Other Accession | P60010, P68136, P68135, P68137, P68134, P68133, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P04751, P68035, P68033, P68032, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709 |
| Reactivity | Zebrafish |
| Predicted | C.Elegans, Drosophila, Xenopus, Chicken, Bovine, Human, Mouse, Rabbit, Rat, Hamster, Horse, Monkey, Pig, Sheep, Yeast |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 41767 Da |
| Antigen Region | 188-215 aa |
| Gene ID | 57934 |
|---|---|
| Other Names | Actin, cytoplasmic 1, Beta-actin-1, Actin, cytoplasmic 1, N-terminally processed, actba, bact, bactin1, bactzf |
| Target/Specificity | This Zebrafish actba antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the central region of zebrafish actba. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Zebrafish actba Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | actba |
|---|---|
| Synonyms | bact, bactin1, bactzf |
| Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. |
| Cellular Location | Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P60710}. Nucleus {ECO:0000250|UniProtKB:O93400} |
| Tissue Location | Skeletal muscle, heart, gill, digestive tissue and brain (PubMed:9987040). Widespread expression throughout the brain, with highest levels in regions where neuronal proliferation is greatest (PubMed:9987040). |
- Primary Antibodies
- Crown Antibodies
- Antibody Collections
- Zebrafish