Rabbit follicle-stimulating hormone,FSH ELISA Kit
Referencia CSB-E06918Rb-24T
embalaje : 24T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
follicle-stimulating hormone,FSH
Associated names
FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
Abbreviation
FSH
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 mIU/mL-100 mIU/mL
Sensitivity
0.39 mIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
Recovery
| The recovery of rabbit FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-105 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rabbit FSH ELISA Kit was designed for the quantitative measurement of Rabbit FSH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL.
Citations
- Alabi QK.et al,J Diet Suppl,2017
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links
KEGG:
STRING:
UniGene: