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> Rabbit Mouse IgG3 (subclass specific), conjugated with TRITC Antibody

Rabbit Mouse IgG3 (subclass specific), conjugated with TRITC Antibody

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Referencia orb21361-1ml

embalaje : 1ml

Marca : Biorbyt


Rabbit Mouse IgG3 (subclass specific), conjugated with TRITC Antibody

SKU: orb21361
This product can be custom made

Description

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to Mouse IgG3, subclass specific. In immunocytochemical and immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG3 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.

Research Area

Immunology & Inflammation

Images & Validation

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Rabbit Mouse IgG3 (subclass specific), conjugated with TRITC Antibody
ReactivityMouse

Key Properties

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Antibody TypeSecondary Antibody
HostRabbit
ClonalityPolyclonal
ConjugationTRITC

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceTRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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