General Info
| Applications: | CLIA |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description: | This rat GzmsB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | Gzmb |
| Gene ID: | 171528 |
| Uniprot ID: | GRAB_RAT |
| Specificity: | This kit recognizes Rat GzmsB in samples. No significant cross-reactivity or interference between Rat GzmsB and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. |
| Protein Name | Granzyme BFragmentinNatural Killer Cell Protease 1Rnkp-1 |
| Database Links | Reactome: R-RNO-5620971Reactome: -RNO-75108 |
| Cellular Localisation | SecretedCytolytic GranuleDelivered Into The Target Cell By Perforin |
| Alternative CLIA Names | Granzyme B CLIA kitFragmentin CLIA kitNatural Killer Cell Protease 1 CLIA kitRnkp-1 CLIA kitGzmb CLIA kit |
| Specificity | This kit recognizes Rat GzmB in samples. No significant cross-reactivity or interference between Rat GzmB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 98-110 | 104 |
| EDTA plasma(n=8) | 95-107 | 101 |
| Cell culture media(n=8) | 89-104 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 23.04 | 76.55 | 245.14 | 24.69 | 76.85 | 261.61 |
| Standard deviation | 2.86 | 7.49 | 25.94 | 3.04 | 6.41 | 16.19 |
| CV (%) | 12.41 | 9.78 | 10.58 | 12.31 | 8.34 | 6.19 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance