600227 | Rat Leptin Clinisciences

Recombinant Active Protein

Cat. No.

600227

Unit

1 mg

Description

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiological regulation of body weight, leptin has a variety of other physiological and pathological functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Rat Leptin produced in E. coli is a single non-glycosylated polypeptide chain containing 147 amino acids with a MW of 16,240 Da.

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Datasheet

Format

Each vial contains 1 mg protein lyophilized in 0.1% TFA. Reconstitute with 1 ml sterile deionized water for a final concentration of 1 mg/ml.

Alternate Names

leptin; obesity factor; obese protein; LEP

Accession No.

P50596

16240 Da

Sequence

MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

rRLeptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependent stimulation of Human OB-R transfected mouse BaF3 indicator cells is 0.06 – 0.35 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Rat Leptin

Referencia 600227

Marca : Abbiotec

Spec Container