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> Rat Myxovirus Resistance 1 (MX1) ELISA Kit

Rat Myxovirus Resistance 1 (MX1) ELISA Kit

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Referencia orb782052-48Tests

embalaje : 48T

Marca : Biorbyt


Rat Myxovirus Resistance 1 (MX1) ELISA Kit

SKU: orb782052
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Description

The Rat Myxovirus Resistance 1 (MX1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Myxovirus Resistance 1 (MX1) in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.121 ng/mL.

Research Area

Infectious Disease & Virology

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MX1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityRat
Sample Typestissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.121 ng/mL
Concentration20 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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IFI-78K ELISA Kit, IFI78 ELISA Kit, MX ELISA Kit, MxA ELISA Kit, Interferon-Inducible Protein p78 ELISA Kit, Interferon-regulated resistance GTP-binding protein MxA ELISA Kit

Quick Database Links

UniProt
P18588

UniProt Details

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No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol