Rat soluble E-selectin,sE-selectin ELISA Kit
Referencia CSB-E07996r-96T
embalaje : 96T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
selectin E
Associated names
Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
78 pg/mL-5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-108 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 89-100 | |||||
| 1:40 | Average % | 84 | ||||
| Range % | 80-88 | |||||
Recovery
| The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 93-105 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
E-selectin (SELE) is a cell adhesion molecule belonging to the selectin family of glycoproteins that plays an important role in leukocyte recruitment during inflammatory responses. This transmembrane protein is primarily expressed on activated endothelial cells and mediates the initial rolling and tethering of neutrophils, monocytes, and other immune cells to the vascular endothelium. E-selectin expression is rapidly upregulated in response to inflammatory cytokines such as TNF-α and IL-1β. This makes it a valuable biomarker for endothelial activation and vascular inflammation in various...
Citations
- Chernukha IM.et al,Anim Sci J,2018
- Secher N.et al,Acta Anaesthesiol Scand.,2017
- H. Guumll Otlu. et al,Biological Rhythm Research,2016
- Turan GA. et al,Arch Gynecol Obstet,2015
- Troelsen TT.et al,Acta Anaesthesiol Scand.,2015
- T. T. TROELSEN et al,Anesthesia & Pain Management,2015
- Secher N. et al,Microcirculation,2015
- Yamakawa K et al,PLoS One,2013
- Tomada I et al,Age (Dordr),2013
Customer Reviews and Q&A
Average Rating:
Sample type: Serum
Sample species: Rat
Sample dilution: No dilution
Review: This figure shows the quadratic fitting curve of rat serum detection with r=0.9951, indicating excellent fitting degree and high adherence between data points and the curve. As the X-axis value increases, the Y-axis response value shows a significant increasing trend, and the detection...
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Target Background
Function
(From Uniprot)
(From Uniprot)
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Gene References into Functions
- The e-selectin/biliverdin reductase pathway participates in the inhibitory process of hypoxia in pulmonary artery endothelial cell apoptosis which is mediated by the mitochondrial-dependent apoptosis pathway.
- Decrease in uterine pinopodes and L-selectin ligand MECA-79 expression during uterine receptivity period by testosterone may cause failure of blastocyst implantation.
- E-selectin blockade similarly inhibits the migration of activated CCR4(negative) and CCR4(positive) CD4 T cells to dermal inflammation.
- The expression of E-selectin is significantly increased in lung tissue of ovariectomized rats, an effect prevented by estradiol but not by progesterone treatment.
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Selectin/LECAM family
Database Links
KEGG:
STRING:
UniGene: