Recombinant Human M-CSF (CHO)

Biological Activity

Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers.

ED50 < 5.0 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 2.0 × 10⁵ units/mg.

Chemical Information

Solubility (25°C)	Reconstitute in ddH₂O or PBS up to 100 μg/ml
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] John A Hamilton. Nat Rev Immunol. Colony-stimulating factors in inflammation and autoimmunity