Recombinant TNF alpha (Tumor necrosis factor alpha), Human, Animal-Free
Referencia orb1179014-20ug
embalaje : 20ug
Marca : Biorbyt
Recombinant TNF alpha (Tumor necrosis factor alpha), Human, AF
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Recombinant TNF alpha (Tumor necrosis factor alpha), Human, Animal-Free
SKU: orb1179014
Description
Research Area
Cancer Biology; Immunology & Inflammation
Images & Validation
−| Tested Applications | Cell Culture, ELISA |
|---|
Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Origin | Human |
| Biological Activity | Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <0.2 pg/mL. The specific activity of recombinant human TNF alpha is approximately >9 x 109 IU/mg. |
| Reactivity | Human |
| Tag | His-tag at the N-terminus |
| Endotoxins | <0.1 EU per 1 μg of the protein by the LAL method. |
| Purity | >98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| Protein Sequence | MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL with polyhistidine tag at the C-terminus. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | The protein was lyophilized from a solution containing 1X PBS, pH 8.0. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin, TNS_x0002_F1A
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Datasheet
Product Information
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Recombinant TNF alpha (Tumor necrosis factor alpha), Human, Animal-Free (orb1179014)
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