RH FGF-10
Referencia 100-183-5UG
embalaje : 5UG
Marca : FUJIFILM
Recombinant Human FGF-10
Fibroblast growth factor 10 (FGF-10) is a growth factor that is important during embryonic development, especially during lung, limb, brain, heart, and kidney morphogenesis. FGF-10 is expressed in mesenchymal cells and facilitates epithelial-mesenchymal signaling through binding the epithelially expressed FGF receptor 2b (FGFR2b). FGF-10 also functions as a mitogen for keratinizing epidermal cells, and induces the migration and invasion of cancer cells.
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SDS-PAGE Showing a Single Prominent Band at 20.0 kDa
Figure 1. rh FGF-10 (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 20.0 kDa (+)
Cell-Based Proliferation Assay
Figure 2. Recombinant Human FGF-10 activity induces proliferation in 4BMR5 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | Keratinocyte growth factor 2 |
| Accession Number | O15520 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 19.3 kDa (170 aa) |
| AA Sequence | MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEIGVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | 4MBr-5 cell proliferation | ≤ 200 ng/mL; ≥ 5.0 x 103 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.