RH SCF
Referencia 100-04-10UG
embalaje : 10UG
Marca : FUJIFILM
Stem cell factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. While human SCF shows no activity on murine cells, murine and rat SCF are active on human cells.
Also available as a GMP product: CTGrade GMP rh SCF
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band ~15 kDa
Figure 1. rh SCF (1 μg) was resolved with SDS-PAGE under reducing (R) conditions and visualized by InstantBlue staining showing a prominent band at ~15 kDa.*
*Predicted MW is 18.6 kDa, but runs on SDS-PAGE at ~15 kDa.
Cell-Based Proliferation Assay
Figure 2. Recombinant Human SCF induced proliferation of TF-1 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | c-Kit Ligand, KL, Steel Factor, MGF |
| Accession Number | P21583 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 18.6 kDa (165 aa) |
| AA Sequence | MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 97% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | TF-1 cell proliferation | ≤ 15 ng/mL; ≥ 6.7 x 104 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
