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> SOD1 (UbetaB) Antibody

SOD1 (UbetaB) Antibody

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Referencia ASM10465-STR-100

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  • ICC/IF - SOD1 (UbetaB) Antibody ASM10465
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (ASM10465). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (ASM10465) at 1:110 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD1 (UBB) Antibody. (C) Composite.
  • WB - SOD1 (UbetaB) Antibody ASM10465
    Western blot analysis of Human, Rat, Mouse Hela cells, Brain, Lung showing detection of ~18 kDa SOD1 (UBB) protein using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (ASM10465). Lane 1: Molecular Weight Ladder. Lane 2: Human HeLa Cell Lysates. Lane 3: Rat Brain. Lane 4: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (ASM10465) at 1:1000. Predicted/Observed Size: ~18 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P00441
Other Accession CAG46542
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human SOD1 (UbetaB) Polyclonal
Target/Specificity Recognizes a conformation specific epitope where the beta barrel is unfolded.
Other Names USOD Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 unfolded beta barrel region Antibody
Immunogen N-terminal region of SOD1, unfolded beta barrel region
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-205 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm